Фосфорилирование - это присоединение фосфатной (PO4) группы к белку или другой органической молекуле. Фосфорилирование активирует или дезактивирует многие ферменты белков, вызывая или предотвращая механизмы развития таких заболеваний, как рак и диабет. Эта книга показывает, как с помощью масс-спектрометрии определить, был ли белок правильно модифицирован добавлением фосфатной группы. Она также предоставляет подробную пошаговую методологию подготовки фосфопротеомных образцов, анализа масс-спектрометрических данных и их интерпретации. Кроме того, в ней описано использование биоинформатических интернет-инструментов, таких как инструмент генной онтологии Blast2GO, который помогает понять и интерпретировать сложные данные, собранные в этих исследованиях.
Written by world-renowned experts dedicated to their research into the powerhouse of connections between proteins and life’s pathologies, this volume guides readers through the application of proteomics in phosphopeptides analysis and identification. Explaining the challenging process of developing a new method to reveal the recognition sites of phosphorylation is a determiner in chronic disease research and dicussed in depth and beautiful language. As readers gain knowledge of methods that elucidate changes of protein functioning, they will have the tools to begin revoalutionizing the fine print of our understanding of dysfunctional cells and its lives-altering consequences in disease states. By understanding the full breadth of President’s Action Plan to combat cancer- related disparities and the extent of elements that drive them, researchers, clinicians, students, and laboratory technicians seeking to better localize issues and develop the necessary analytical practices needed to combat them will find much here. Special interest readers will garner perceptions and avenues of thought not only enlightening in their fervor, but also forming the foundation-lifting for their considerable advancement encompassed within. A stunning addition to the library of any researcher dedicated to proteomics/phosphoproteomics research, with this thorough yet informative compilation, readers of all academic echelons shall be well-padded with knowledge and pursue new worlds of inquiry, opening windows before them and revelations throughout.
Электронная Книга «Proteomics of Biological Systems. Protein Phosphorylation Using Mass Spectrometry Techniques» написана автором Bryan Ham M. в году.
Минимальный возраст читателя: 0
Язык: Английский
ISBN: 9781118137017
Описание книги от Bryan Ham M.
Phosphorylation is the addition of a phosphate (PO4) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. This book shows how to use mass spectrometry to determine whether or not a protein has been correctly modified by the addition of a phosphate group. It also provides a combination of detailed, step-by-step methodology for phosphoproteomic sample preparation, mass spectral instrumental analysis, and data interpretation approaches. Furthermore, it includes the use of bioinformatic Internet tools such as the Blast2GO gene ontology (GO) tool, used to help understand and interpret complex data collected in these studies.
